ABSTRACT
ObjectiveChemical components in ethanol extract of Cyclocarya paliurus dried leaves were analyzed by ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry （UHPLC-Q-TOF/MS）. MethodAn Agilent Poroshell 120 EC-C18 column （3.0 mm×100 mm， 2.7 μm） was used with 0.1% formic acid aqueous solution （A）-acetonitrile （B） as the mobile phase for gradient elution （0-26 min，2%-18%B； 26-60 min， 18%-72%B； 60-70 min， 72%-100%B； 70-71 min， 100%-2%B； 71-72 min， 2%B）， and the flow rate of 0.4 mL·min-1 and injection volume of 3 μL. The electrospray ionization （ESI） was used in positive and negative modes， and detection range was m/z 50-1 100. The collected data were processed by Agilent MassHunter workstation. According to the retention time and MS information of each compound， combined with existing literature and MS database information， the compounds were identified and analyzed for the fragmentation rule. ResultA total of 52， 55 components were identified in the positive and negative ion modes， respectively. Among them， 14 flavonoids， 3 triterpenoids， 15 organic acids and 20 other compounds were identified under positive ion mode， while 18 flavonoids， 9 triterpenoids， 18 organic acids and 10 other compounds were identified under the negative ion mode. By summarizing the positive and negative ion modes and removing the common compounds， 87 compounds were identified， including 22 flavonoids， 27 organic acids， 11 triterpenoids and 27 other compounds. ConclusionUHPLC-Q-TOF/MS can be used to quickly analyze the chemical constituents in C. paliurus dried leaves. 1-Kestose and 18β-glycyrrhetinic acid and other components related to hypoglycemic activity of this herb are identified for the first time， which can provide reference for clarifying the pharmacodynamic substance basis of C. paliurus dried leaves.
ABSTRACT
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS: The Angilent Zorbax,Extend C-18 column(4.6 mm×250 mm,5μm) was used with methanol-water (containing 0.4% phosphonic acid)(50: 50) as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS : The method had the good linear relationship within the range of 0.04-0.61 μg for luteolin and within the range of 0.20-3.01 μg for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence (264.8 μg/g,2 746.3 μg/g) in August,respectively.CONCLUSION: The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.
ABSTRACT
OBJECTIVE:To analyze the chemical constituents of the essential oil extracted from Mosla chinensis seeds. METHODS: The essential oil was extracted from Mosla chinensis seeds by steam distillation method and identified by GC-MS, and its relative content was determined using normalization method. RESULTS: A total of 20 chromatographic peaks were detected and 17 chemical compounds were identified, which made up 99.06% of the total essential oil content. The major chemical constituents were carvacrol(57.01%), thymol(30.72%), thymol acetate(2.56%), carvacrol acetate(2.14%) and ?-caryophyllene(1.62%). CONCLUSION: The results in our study provides scientific basis for further exploitation and utilization of Mosla chinensis and serves as reference for establishing quality standard of Mosla chinensis herbal medicine.
ABSTRACT
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS:The Angilent Zorbax,Extend C-18 column(4.6 mm?250 mm,5 ?m)was used with methanol-water(containing 0.4% phosphonic acid)(50 ∶50)as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS:The method had the good linear relationship within the range of 0.04-0.61 ?g for luteolin and within the range of 0.20-3.01 ?g for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence(264.8 ?g/g,2 746.3 ?g/g)in August,respectively.CONCLUSION:The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.